the effect of il-22 and il-28 in induction of type 1 regulatory t (tr1) cells.
نویسندگان
چکیده
cytokines have been introduced as critical inducers in the development of th subpopulations.cytokines like il-10 are involved in inducing regulatory t cells such as type 1 regulatory t (tr1) cells cells. il-22 is a member of il-10 family of cytokines, and il-28a is a member of ifn-γ family. in this study, cord blood mononuclear cells (cbmc) from normal healthy individuals were isolated by ficoll and then naïve t cells were purified by cd4+cd25+ regulatory t cell isolation kit. the effect of these two cytokines on production of il-5, tgf-β, il-10, il-4 and ifn-γ cytokines from cord blood t cells was investigated to identify tr1 cells as well as th1 and th2 polarization. flow cytometric analysis showed that il-28a and il-22 were not effective in expression of il-5 and tgf-β either alone or in synergy, but in view of il-10, il-4 and ifn-γ, the results showed that il-22 increased il-10 and il-4 but had a decreasing effect on ifn-γ. the results showed that il-28a was not effective in increasing or decreasing the level of il-10, il-4 and ifn-γ. therefore, according to these results, il-22 and il-28a were not effective in inducing tr1 cells.
منابع مشابه
IL-10-producing type 1 regulatory T cells and allergy.
As an important subset of regulatory T (Treg) cells, IL-10-producing type 1 regulatory T cells (Tr1), have some different features to thymic-derived naturally occurring CD4+CD25+Foxp3+ Treg cells(nTreg cells). Similar to nTreg cells, Tr1 also play important roles in the control of allergic inflammation in several ways. There is a fine balance between Tr1 and Th2 responses in healthy subjects. S...
متن کاملthe investigation of the relationship between type a and type b personalities and quality of translation
چکیده ندارد.
IL-21 induces IL-22 production in CD4+ T-cells
Interleukin (IL)-22 produced by innate lymphoid cells (ILCs) and CD4+ T cells plays an important role in host defence and mucosal homeostasis, thus it is important to investigate the mechanisms that regulate IL-22 production. We investigated the regulation IL-22 production by CD4+ T cells. Here we show that IL-21 triggers IL-22, but not IL-17 production by CD4+ T cells. STAT3, activated by IL-2...
متن کاملevaluation of the effect of il-22 on human cord blood cd4+ t cells
il-22 is a member of il-10 cytokine family which is believed to play an important role in inflammatory responses. il-22 has similarities with il-10 including conserved sequences with il-10. il-22 receptor is also comprised of two chains known as l-22r1 and l-10r2; supporting the speculation that the two cytokines may have similar effects. the aim of this study was to shed some light on the biol...
متن کاملInduction of Interleukin-22 (IL-22) production in CD4+ T Cells by IL-17A Secreted from CpG-Stimulated Keratinocytes
BACKGROUND Interleukin-17A (IL-17A) is mainly secreted from Th17 cells that are activated by various stimuli including CpG oligodeoxynucleotide, a Toll-like receptor 9 (TLR9) ligand. Recently, it has been demonstrated that keratinocytes play an important role in the pathogenesis of psoriasis. OBJECTIVE To investigate the potential role of keratinocytes, we examined whether TLR9 ligand CpG ind...
متن کاملIL-27-Induced Type 1 Regulatory T-Cells Produce Oxysterols that Constrain IL-10 Production
The behaviors of lymphocytes, including CD4+ T helper cells, are controlled on many levels by internal metabolic properties. Lipid metabolites have recently been ascribed a novel function as immune response modulators and perturbation of steroids pathways modulates inflammation and potentially promotes a variety of diseases. However, the impact of lipid metabolism on autoimmune disease developm...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۱۴، شماره ۲، صفحات ۱۵۸-۱۶۷
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023